Sequencing of patient-derived colorectal cancer organoids
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Sequencing of patient-derived colorectal cancer organoids to determine the genetic background of human cultures for treatment studies.
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2023-04-13
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VDR ChIP-Seq on colonic human organoids
Genome-wide maps of VDR binding sites in colonic organoids estimulated with calcitriol Overall design: Three independent VDR ChIP-Seq analysis of 3D stem cell enriched cultures of two different patien
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Whole-exome sequencing (WES) to assess genomic concordance between an adenoma/adenocarcinoma colonoid culture 14-881 and the source patient tissue. Whole-exome sequencing (WES) to assess genomic concordance between an adenoma/adenocarcinoma colonoid culture 14-881 and the source patient tissue
A patient-derived epithelium-only colon rectal organoid, also referred to as a colonoid, was generated from an adenoma (associated with a resection surgery of an invasive moderately differentiated col
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Additional file 19: of A systematic analysis of genomics-based modeling approaches for prediction of drug response to cytotoxic chemotherapies
MAD values for NLSVR CTR2 models discussed in section E. (XLSX 26 kb)
Figshare2019-06-17 更新20
FGF Signaling in the Self-Renewal of Colon Cancer Organoids. FGF Signaling in the Self-Renewal of Colon Cancer Organoids
With their ability to self-renew and simultaneously fuel the bulk tumor mass with highly proliferative tumor cells, cancer stem cells (CSC) are supposedly driving cancer progression. However, the CSC-
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Targeted sequencing of IDH1 and IDH2 genes was performed using bone marrow specimens from matched diagnosis and relapsed AML patients with IDH baseline mutation before or after IDHmut inhibitor treatment. gDNA was extracted from the bone marrow clot sections using the QuickExtract FFPE DNA extraction kit (Epicenter Biotechnologies) following the manufacturer's instructions. The exon sequences of IDH1 and IDH2 genes were PCR amplified. The DNA fragments for each exon were purified with QIAquick spin columns (Qiagen), mixed at equal molarity, and fragmented using Covaris acoustic shearing following the manufacturer’s instructions. Sonicated DNA was processed for library generation using NEBNext Ultra II DNA Kits (New England Biolabs) following the manufacturer’s protocol. Libraries with different index sequences were quantified, pooled, and sequenced on an Illumina Nextseq500 system using the 75bp high output sequencing kit.. Targeted sequencing of IDH1 and IDH2 genes in AML samples
Acquired resistance to allosteric inhibition of cancer-associated IDH mutations has been described through mutations to restore the production of oncometabolite R-2HG, yet the complete repertoire of I
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