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Gene expression data of fibroblasts with differential Gli1 expression in the colon.

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE105069
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The morphogen Indian Hedgehog is expressed by the intestinal epithelium and signals in paracrine manner to fibroblasts by activating trascription factor Gli1. ZsGreen is used to track Gli1+ cells. To describe different fibroblast subpopulations we used gp38 as a general marker and Sca1 as a marker expressed in some of gp38+ cells. Fibroblasts from colon tissue from Gli1CreERT2-ZsGreen mice were isolated and Epcam-CD45-CD31-gp38+ fibroblasts were flow-sorted based on expression of Sca1 and Gli1ZsGreen generating 4 fibroblast subpopulations from 4 different mice: 1.Sca1+Gli1ZsGreenhigh, 2.Sca1+Gli1ZsGreendim, 3.Sca1+Gli1ZsGreen- and 4.Sca1-Gli1ZsGreen-.
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2021-07-25
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