Gene expression data of fibroblasts with differential Gli1 expression in the colon.

The morphogen Indian Hedgehog is expressed by the intestinal epithelium and signals in paracrine manner to fibroblasts by activating trascription factor Gli1. ZsGreen is used to track Gli1+ cells. To describe different fibroblast subpopulations we used gp38 as a general marker and Sca1 as a marker expressed in some of gp38+ cells. Fibroblasts from colon tissue from Gli1CreERT2-ZsGreen mice were isolated and Epcam-CD45-CD31-gp38+ fibroblasts were flow-sorted based on expression of Sca1 and Gli1ZsGreen generating 4 fibroblast subpopulations from 4 different mice: 1.Sca1+Gli1ZsGreenhigh, 2.Sca1+Gli1ZsGreendim, 3.Sca1+Gli1ZsGreen- and 4.Sca1-Gli1ZsGreen-.