Global studies of microbial diversity on soil

Through next-generation sequencing (NGS) approach, was possible the study of a large fraction of soil species by analyzing DNA/RNA sequences. A metagenomic greenhouse study was carried out to identify the effects of the root-knot nematode Meloidogyne incognita (RKN) and chemical treatments on the tomato rhizosphere bacterial biodiversity. A RKN-free organic soil planted with tomato was Studied. Treatments included RKN inoculated plants, with or without a nematicidal (fenamiphos), and treated/untreated RKN-free soils as controls. The total RNAs, extracted and sequenced (Illumina MiSeq System) using the V4 - 16S rRNA region, allowing identification of> 97% of taxa. NGS data at finer scales showed taxonomic groups or generates directly affected by nematodes and chemical treatments