Transmission of Arsenophonus from single infected females vs mixed infected/uninfected groups

Two treatments: a) Single Pachycrepoideus vindemmiae females infected with Arsenophonus were allowed to oviposit on D.melanogaster pupae within a vial. F1 progeny were then tested for Arsenophonus presence via PCR (with QC on insect to verify amplifiability). b) Groups of 2 infected and 2 uninfected P. vindemmiae females were allowed to oviposit on D.melanogaster pupae within a vial. F1 progeny were then tested for Arsenophonus presence via PCR (with QC on insect to verify amplifiability).

Column A: Sample #

Colimn B: Treatment (single female or group lay as defined above)

Column C: Vial replicate number

Column D: QC PCR results on insect DNA (1 = amplifiable 0= fail)

Column E: PCR assay results for Arsenophonus presence (1 = present, 0 = absent)

Column F: PCR plate/well

Column G: Notes, e.g. if sample was rerrun when first assay was ambiguous.