Leaf disc assay.

Three sets of twelve leaf discs each were infiltrated using Agrobacterium containing a construct encoding one of the three model proteins, DsRed, phl p 1 or M12. After the infiltration and a five-day incubation period (25°C, 16/8 h light/dark cycle, 7 klux), nine leaf discs from each set were pooled and extracted simultaneously. For phl p 1 and M12, the recombinant protein concentrations were determined via ELISA. The DsRed concentration was determined by measuring the fluorescence in the cell extract. All values were normalized to the TSP content, which was determined via Bradford assay. The max/min ratio indicates the difference between the highest and lowest concentration measured for each model protein.