Bioindication diatomées : comparaison microscopie/barcoding ADN. Données bruts Fastq 2016 + 2017, Test des différentes stratégies bioinfo sur données 2016. Projet AFB numéro 15000239 / A30

These data were generated as part of the DREAL project from 2016 to 2017 for the assessment of the Diatom Biological Index (DBI) required by the Water Framework Directive (WFD) and the comparison between microscopy and DNA barcoding methods. Samples were collected by french Water Agencies and OFB laboratories (Adour-Garonne, Rhin-Meuse, Loire-Bretagne, Rhône-Méditerannée-Corse, Seine-Normandie, Artois-Picardie). DNA was extracted using the NucleoSpin® Soil kit (Macherey-Nagel). Extracted DNA was amplified by PCR using RbcL primers (3FW + 2RV). FastQ files were obtained after DNA sequencing based on the Illumina MiSeq technology with the v3 reagent kit (2 x 250pb) at the GeT-Plage platform (Toulouse, France). This directory contains the following files : DREAL_2016_Run0.tar.gz : all samples FastQ files 2016 (284 x 2 replicates) DREAL_2017_Run1.tar.gz : all samples FastQ files from the first run 2017 (200 x 2 replicates) DREAL_2017_Run2.tar.gz : all samples FastQ files from the second run 2017 (171 x 2 replicates) Metadata_DREAL_2016-17.csv : contains all the data available for each samples corresponding to the names of FastQ files