A high-quality genome assembly for a desert-adapted rodent, Merriam’s kangaroo rat (Dipodomys merriami)

Merriam’s kangaroo rat (Dipodomys merriami) is a member of a unique family of primarily desert-adapted North American rodents (Heteromyidae). Of the 20 species in the genus, D. merriami is one of the most wide-ranging and ecologically flexible, inhabiting desert scrub, grassland, sagebrush steppe, and juniper-piñon woodland in the southwestern deserts of the United States and Mexico. We present a de novo reference genome for D. merriami generated from PacBio HiFi long-read and Omni-C chromatin proximity sequencing as a part of the California Conservation Genomics Project. The primary pseudo-haplotype assembly comprises 3,110 scaffolds, with a contig N50 of 8.6 Mb, scaffold N50 of 49.1 Mb, and a total length of 3.57 Gb. Further, a BUSCO completeness score of 97.8% suggests that the assembly is highly complete. The reference genome sequence is available on NCBI under accession GCA_023159225.1. Here, we share supplementary data, including a a mitochondrial genome assembly and a basic protein-coding gene annotation lifted over from Dipodomys spectabilis, the closest relative of D. merriami with an annotated genome.