Transcriptomes of evolved Pseudomonas putida KT2440 on non-native sugars

Efficient utilization of lignocellulosic biomass-derived sugars is essential to improve the economics of biorefinery. While Pseudomonas putida is a promising microbial host, its usage is limited because this strain cannot utilize xylose or galactose as a sole carbon source. To address this issue, we heterologously introduced a xylose utilizing gene (xylD) from Caulobacter crescentus and a galactose operon (galETKM) from E. coli MG1655. To improve the utilization further, we evolved the engineered strains in minimal medium conditions. After the evolution, they acquired better fitnesses on the non-native sugars. To understand transcriptional changes after the evolution, the transcriptomes of few evolved isolates were analyzed. 4 evolved KT2440-xylD isolates and 3 evolved KT2440-galETKM isolates were grown on the minimal media supplemented with either xylose or galactose as a sole carbon source. The cultures were conducted with two biological duplicates.