Osteocyte Connexin 43 Hemichannels Influence Bone Marrow Mesenchymal Stromal Cell Fate through Prostagladin E2. Osteocyte Connexin 43 Hemichannels Influence Bone Marrow Mesenchymal Stromal Cell Fate through Prostagladin E2

Inhibiting Connexin 43 (Cx43) hemichannels in osteocytes led to a significant increase in adipogenesis and a decrease in osteogenesis in bone marrow mesenchymal stromal cells. Overall design: Bone marrow mesenchymal stromal cells (BM-MSCs) were isolated from three groups: the WT group and two transgenic mouse models, R76W (inhibiting osteocyte Cx43 gap junctions) and Δ130–136 (blocking osteocyte Cx43 hemichannels and gap junctions). Subsequently, single-cell RNA sequencing was conducted on the sorted BM-MSCs to unravel their transcriptomic profiles.