Identification of STAT6 Phosphorylation
收藏国家人口健康科学数据中心2026-06-06 收录
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https://www.ncmi.cn/phda/dataDetails.do?id=CSTR:17970.11.A001G.202602.45.V1.0
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To identify potential phosphorylation sites of STAT6, RAW 264.7 cells were overexpressed with STAT6-HA. 48 hours after transfection, cells were treated with 10 μM etoposide for 2 h, then lysed for immunoprecipitation using anti-HA beads. Immunoprecipitates were separated by SDS–PAGE and then Coomassie blue staining was carried out. Bands of interest were cut from the gel and then in-gel digestion was performed using sequencing grade-modified trypsin. The peptides were analyzed by liquid chromatography–tandem mass spectrometry (LC–MS/MS) with nano-LC combined with Orbitrap Q Exactive mass spectrometer
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北京蛋白质组研究中心创建时间：
2026-02-11
