Influence of the interval between toxin and antitoxin injections on survival rate (values in brackets).

The indicated times correspond to the interval between toxin and antitoxin injections.
At T = 0 5 estimated MDL50 of botulinum toxin type A1 (0.5 ml) were injected i.p. into all mice. The antitoxin was then injected i.p. into mice after 0, 2, 4 or 6 hours. An amount of antitoxin (2.5 µg/mouse) equivalent to 100 times the dose which protected 100% of the mice challenged with 5 estimated MLD50 in the co-injection experiment was used. Botulism symptoms and mortality were recorded for 4 days. Results are expressed as the number of dead mice versus the total number of challenged mice.