Lung Epithelial Response to Cigarette Smoke and Modulation by the Nicotinic Alpha 7 Receptor

We report the impact of side-stream cigarette smoking on baseline tracriptional status of enriched epithelium from the distal lung of both male and female control mice or mice harboring a mutation in the nicotinc alpha7 recptor that selectivley diminshes the calcium current (E260A). Mice (male or female) of each nicotinic recptor alpha7 genotype (Control (c) or mutant (E260A)) were exposed to side-stream cigarette smoke 5 days per week for four months. The distal lung epithelium was enriched and poly-adenylated strand-specific RNA-Seq libraries using Illumina TruSeq stranded mRNA were preared for analysis.本研究报道了侧流香烟烟气（side-stream cigarette smoking）对雌雄对照小鼠，以及携带可选择性减弱钙电流的烟碱型α7受体（nicotinic alpha7 receptor）E260A突变体小鼠的远端肺富集上皮细胞的基线转录状态的影响。实验中，不同烟碱型α7受体基因型（野生型对照(c)与突变型(E260A)）的雌雄小鼠，每周暴露于侧流香烟烟气5天，持续4个月。随后，我们富集了小鼠远端肺上皮细胞，并采用Illumina TruSeq链特异性mRNA建库体系制备了聚腺苷酸化链特异性RNA测序（RNA-Seq）文库，用于后续分析。