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Specificity constants of TGase-mediated transamidation.

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https://figshare.com/articles/dataset/Specificity_constants_of_TGase-mediated_transamidation_/6393638
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0.2–0.8 μM mRuby2-Qtag test proteins and 5 mM Gly-OMe were reacted in the presence of 0.1 U TGase. Initial rates were measured over 20 min, using the GDH-coupled assay. Specificity constants were determined by fitting initial rate data to the Michaelis-Menten equation (see Materials and methods).
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2018-05-30
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