R.palustris: cbbT1 overexpression and cbbT2 overexpression. Rhodopseudomonas palustris

Transcriptional profiling of Rhodopseudomonas palustris (R. palustris) comparing cbbT1 over-expressing strain with cbbT2 over-expressing strain. Goal was to discriminate the molecular mechanisms between transketolase I (cbbT1) and transketolase II (cbbT2). R.palustris is a purple non-sulfur anoxygenic phototrophic bacterium and transketolase (cbbT1 and cbbT2) is a key enzyme involved in the CBB cycle. Here, we investogated the functions of transketolase isoforms I (cbbT1) and II (cbbT2) in R. palustris through transcriptional profiling and other functional assays. Overall design: Four-condition experiment, cbbT1 over-expressing cells, cbbT2 over-expressing cells, Negative control strain with empty plasmid, Wild type strain.