Isoform sequencing of barley (Hordeum vulgare), using the reference cultivar 'Morex'.

Transcriptome sequencing with short read technology usually involves either de novo assembly or mapping of the reads to a reference sequence. Both of these approaches can result in bioinformatics artefacts that lead to the output of fragmented transcripts. To overcome this problem we chose a Pacific BioSciences IsoSeq approach which can theoretically yield full-length transcripts, without the need for assembly or read mapping. We applied this approach in barley (Hordeum vulgare), using the reference cultivar 'Morex'.