The function of 3-Indolepropionic acid(IPA) in intestinal homeostasis

Intraepithelial immune cells and intestinal epithelial cells from the small intestine of mice were analyzed for the investigation of the impact of IPA. C57BL/6J male mice (8 weeks) were fed on high-fat diet (HFD) + l-NAME containing either control (CON) or high IPA (562.5 mg IPA/kg diet) for 7 weeks before dissection and small intestines were isolated. Cells from small intestine epithelium layer were sorted for DAPI- live cells and analyzed using 10x Genomics Chromium droplet single cell RNA sequencing (scRNA-seq). Each sample is pooled from two individual mice.