Genome-wide localization of Swi6 and H3-Lys9-me2 in wt and mutant starins of S. pombe

ChIP-on-ChIP experiments were performed to quantitate the genome-wide localization of heterochromatin proteins Swi6 and H3-Lys9-me2 in wild type versus polalpha mutant swi7H4, Pol delta mutant, dts2 and cut4 mutant cut4-993. Our results indicate that the localization of Swi6 and H3-Lys7-me2 at heterochromaitn regions, like centromere, telomere and mating-type is abrogated in the swi7H4, dts2 and cut4 mutants. Agilent S. pombe ChIP-on-chip Microarray, 4X44K (AMADID:015424)