Figure 7 Dataset - Integration of vascular flow in the complete hToC culture

This figure describes the culture timeline for a tendon-on-a-chip model and details relevant cell types and the microfluidic device layout. The dataset includes raw confocal images of live-labeled cells within the tissue chip culture. The prism file includes the final figure layout as well as the raw quantification values for the number of adhered and transmigrated monocytes.The images can be analyzed in a software that enables 3D image analysis, such as Imaris. To quantify fluorescently labeled monocytes in confocal images, the Spots tool in Imaris was used to identify cells based on a specified diameter (10 um). Cells below the membrane were considered to be transmigrated. A fluorescent intensity threshold was determined and kept consistent across images for Spot identification. Quantified data was exported from Imaris and analyzed in GraphPad Prism.This dataset supports Fluid flow impacts endothelial-monocyte interactions in a model of vascular inflammatory fibrosis, published in <i>Scientific Reports</i><i>,</i><i> </i>Vol. 15, Iss. 3227, January 2025.