RNAi clones that render the animals hypersensitive to Cry5B PFT.

aThe gene names were annotated based on WormBase release WS221. All the previously unnamed genes were named either ttm or hpo based on their phenotypes.bWe have published ten genes that upon reduction lead to a Hpo phenotype and knew of one other gene in the p38 pathway (tir-1) that is a strong hpo. Of these 11, three are not in the Ahringer RNAi library (wwp-1, sek-1, pmk-1); one (ire-1), upon RNAi, leads to sickly worms (would have been screened out). Of the remaining seven genes, four have relatively weak to moderate Hpo phenotypes upon knock down (ttm-1, ttm-2, hif-1, and xbp-1) were not found (likely missed due to the lack of sensitivity associated with a genome-wide RNAi experiment). All three of the remaining genes (nsy-1, tir-1, kgb-1) that upon knock down result in a strong Hpo phenotype and are otherwise healthy were identified.cH., M., and D. refer to Homo sapiens, Mus musculus and Drosophila melanogaster, respectively.d, e% survivals are relative to empty vector controls.f√ indicates the RNAi clones further confirmed in another round of quadruplicate liquid RNAi assays as shown in Table S1.g“▴” indicates induction by Cry5B and “▾” indicates repression by Cry5B in 3-hour microarray with a 1.5 fold cutoff. DEG = differentially expressed genes.