Coral thermotolerance retained following year-long exposure to a novel environment

Description of datasets:

HTSeqCounts

Raw HTSeq count data used for downstream RNA sequencing analyses and visualisations including Principal Component Analysis and Differential Gene Expression analysis of the four habitat groups (mangrove to reef, reef to reef, wild mangrove and wild reef).

GO enriched gene results:

GO enriched genes summarised by broad categories of GO Slims for Pocillopora acuta corals originating from a mangrove system vs colonies from an adjacent reef site, translocated to a reef environment for one year. Samples for gene expression were collected during an acute heat stress assay to assess differentially enriched genes between mangrove and reef corals under 36 ºC. Enriched GO terms were grouped into broader functional categories using the “goSlim” function in the GSEABase R package with GOslim generic obo as the reference database to allow for a summary of key functions enriched in groups under heat stress. See manuscript methods for further detail on differential gene expression analysis.

2022-2023 mangrove/reef temperature and pH

Temperature and pH data measured in the Low Isles mangrove lagoon and reef habitat using HOBO MX2510 loggers deployed from February 2022 - February 2023. 

Methylated DNA data

Percent DNA methylation relative to total DNA of mangrove to reef, reef to reef and wild mangrove groups under acute temperature stress during the February 2023 CBASS experiment.

Analysis code.zip

A copy of all R code used in the data analysis of this project