Next Generation RNA sequencing of 30 human liver tissues at different developmental stages (10 each of Fetal, Pediatric and Adult)

miRNAs have emerged as key regulators of gene expression. Here we sequenced mRNA from 30 human liver samples to identify genetic polymorphisms in predicted miRNA binding regions that show allele-specific gene expression. These genetic variants will be functionally tested for miRNA-mediated regulation. Standard methods were used for RNA-sequencing library construction, EXBead preparation, and Next-Generation sequencing, based on the protocol provided for the Life Technologies SOLiD4 system. Briefly, 2μg of total RNA per sample of 30 human liver samples (fetal, pediatric, and adult; n=10) were used for library preparation. The rRNA was depleted using RiboMinus Eukaryote Kit for RNA-Seq.