RNA-seq of Aedes (Ochlerotatus) fluviatilis cell lines to study metabolic and immune interactions in mosquito-Wolbachia symbiosis

In a previous study, we established two novel embryonic cell lines from the mosquito Aedes (Ochlerotatus) fluviatilis containing or not the naturally occurring symbiont bacteria Wolbachia, which were called wAflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatment, named wAflu1T. RNA-seq analyzes were performed aiming to elucidate the symbiont-host interaction in the context of energy metabolism regulation and molecular mechanisms of the immune responses involved.