Comparative proteomic analysis of the moderately resistant and susceptible peanut cultivars during infestation by <i>Cylindrocladium parasiticum</i>
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<i>Cylindrocladium parasiticum</i> Crous, Wingfield & Alfenas, the causal agent of Cylindrocladium black rot of peanut, has led to economic losses in China. However, the response of peanut plant to <i>C. parasiticum</i> is poorly understood. Here, two contrasting peanut cultivars, T09 (<i>C. parasiticum</i>-moderately resistant) and P562 (<i>C. parasiticum</i>-susceptible) were used for comparative analysis of protein profiles in root segment of plant tissues in responses to <i>C. parasiticum</i> infection. Proteomic profiling identified 1647 and 391 differentially accumulated proteins (DAPs) in P562 and T09 relative to uninoculated control, respectively. A total of 350 and 1095 DAPs were identified between P562 and T09 after 9 day(s) post infection (dpi), respectively. Functional categorization showed that <i>C. parasiticum</i>-responsive proteins were mainly involved in catalytic activity and binding functions. KEGG pathway analysis indicated both moderately resistant and susceptible peanut cultivars can regulate gene expression in the phenylpropanoid pathway, terpenoid backbone biosynthesis, SA, and JA pathways to induce defensive genes and protein expression which enhances plant defence or susceptibility capacity. However, MAPK signal pathway was more pronounced in moderately resistant peanut cultivar T09. We also observed an increase of CYP73A100 involved in phenylpropanoid and flavonoid biosynthesis pathways in susceptible peanut ecotype P562, while decrease in moderately resistant interaction. Additionally, there was a marked activation of brassinosteroid biosynthesis in T09, which indicated a possible involvement of activation of plant immune response in moderately resistant responses of peanut to <i>C. parasiticum</i>. This study provides some insights into molecular networks involved in cellular and physiological responses to <i>C. parasiticum</i> in peanut.
寄生柱枝双孢霉（Cylindrocladium parasiticum）Crous, Wingfield & Alfenas是花生圆筒霉黑腐病的致病菌，该病害已在我国造成经济损失。然而，目前人们对花生应对该病原菌的分子应答机制仍知之甚少。本研究选取两个表型差异显著的花生品种：T09（寄生柱枝双孢霉中抗品种）与P562（寄生柱枝双孢霉感病品种），通过比较分析二者根系组织在受该病原菌侵染后的蛋白质表达谱。蛋白质组学分析显示，相较于未接种对照组，感病品种P562与中抗品种T09分别存在1647种和391种差异积累蛋白（differentially accumulated proteins, DAPs）。接种后9天（days post infection, dpi），P562与T09之间分别鉴定到350种和1095种差异积累蛋白。功能分类注释结果表明，响应寄生柱枝双孢霉侵染的蛋白主要参与催化活性与结合功能两类分子功能。京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes, KEGG）通路分析显示，中抗与感病花生品种均可通过调控苯丙烷类通路、萜类骨架生物合成通路、水杨酸（salicylic acid, SA）以及茉莉酸（jasmonic acid, JA）通路的基因表达，诱导防御相关基因与蛋白的表达，从而增强植物的防御或感病能力。但丝裂原活化蛋白激酶（mitogen-activated protein kinase, MAPK）信号通路在中抗品种T09中的富集程度更为显著。研究还发现，参与苯丙烷类与黄酮类生物合成通路的CYP73A100在感病花生品种P562中表达上调，而在中抗品种与病原菌的互作体系中表达下调。此外，油菜素甾醇生物合成通路在T09中被显著激活，这表明该通路的激活可能参与花生对寄生柱枝双孢霉的中抗免疫应答过程。本研究为解析花生应对寄生柱枝双孢霉侵染时的细胞与生理应答分子网络提供了重要参考与新的见解。
