Transcriptomic responses of hornyhead turbot (Pleuronichthys verticalis) to environmentally relevant doses of polybrominated diphenyl ethers or polychlorinated biphenyls

Hornyhead turbot (Pleuronichthys verticalis), a sentinel flatfish species, were intraperitoneally injected with environmentally relevant mixtures of polybrominated diphenyl ethers (PBDEs) or polychlorinated biphenyls (PCBs). After 96 h, fish were sacrificed and liver tissue was collected for gene expression analysis using a custom-designed microarray. Hornyhead turbot (Pleuronichthys verticalis), a sentinel flatfish species, were intraperitoneally injected with a low or high dose of environmentally relevant mixtures of polybrominated diphenyl ethers (PBDEs) (0.5 or 5 µg/g body weight) or a low or high dose of polychlorinated biphenyls (PCBs) (1 or 10 µg/g body weight). After 96 h, fish were sacrificed and liver tissue was collected for chemical and gene expression analyses. Gene expression analysis was performed using a custom-designed Agilent 8 x 15K microarray (n = 5-7 fish/treatment group).