Streptococcus pyogenes Cas9 (SpCas9) and derived enzymes are widely used as components of CRISPR-Cas9 genome editing and engineering platforms. However, they exhibit promiscuous nuclease activity that
*Based on Ensembl BLAT release 60- Nov 2010. **Confirmed by PCR amplification and sequencing across integration sites. †Located within 5 kb upstream or downstream of a known transcription start site.