Brain size predicts bees’ tolerance to urban environments

The rapid conversion of natural habitats to anthropogenic landscapes is threatening insect pollinators worldwide, raising concern on the negative consequences for their fundamental role as plant pollinators. However, not all pollinators are negatively affected by habitat conversion, as certain species find in anthropogenic landscapes appropriate resources to persist and proliferate. The reason why some species thrive in anthropogenic environments while most find them inhospitable remains poorly understood. The cognitive buffer hypothesis, widely supported in vertebrates but untested in insects, offers a potential explanation. This theory suggests that species with larger brains have enhanced behavioural plasticity, enabling them to confront and adapt to novel challenges. To investigate this hypothesis in insects, we measured brains for 89 bee species, and evaluated the association between brain size and habitat preferences. Our analyses revealed that bee species that prefer urban habitats had larger brains relative to their body size than those who prefer forested or agricultural habitats. Additionally, urban bees exhibited larger body sizes and, consequently, larger absolute brain sizes. Our results provide the first empirical support for the cognitive buffer hypothesis in invertebrates, suggesting that a large brain in bees could confer behavioural advantages to tolerate urban environments.

Methods
This dataset contains measurements of brain and body size measurements for bee species captured in Europe and North America. In addition, we include information about their feeding specialisation and the occurrence information downloaded from the Global Biodiversity Information Facility. Finally, all the code and data generated files for processing, analysing, and writing this manuscript are also included.