RNAseq data relating to manuscript: Prior Fc Receptor activation primes macrophages for increased sensitivity to IgG via long term and short term mechanisms.

Macrophages measure the ‘eat-me’ signal IgG to identify targets for phagocytosis. We tested if prior encounters with IgG influence mouse bone-marrow-derived macrophage appetite. IgG is recognized by the Fc Receptor. To temporally control Fc Receptor activation, we engineered an Fc Receptor that is activated by light-induced oligomerization of Cry2, triggering phagocytosis. Using this tool, we demonstrate that subthreshold Fc Receptor activation primes macrophages to be more sensitive to IgG in future encounters. Macrophages that have previously experienced subthreshold Fc Receptor activation eat more IgG-bound cancer cells. Increased phagocytosis occurs by two discrete mechanisms – a short- and long-term priming. Long-term priming requires new protein synthesis and Erk activity. These long-term transcriptional changes show an increase in immune response genes and a reprogramming of macrophages towards an M2 polarization. Short-term priming does not require new protein synthesis and corr..., , , # RNAseq data

Data are RNAseq FASTQ files relating to figures 5a,b; S4a,b in the manuscript entitled Prior Fc Rceptor activation primes macrophages for increased sensitivity to IgG via long term and short term mechanisms. Data were analyzed using code initially described in Atkins el al 2024 doi: [10.1021/acsbiomaterials.3c01079](https://doi.org/10.1021/acsbiomaterials.3c01079 \"DOI URL\")

## Description of the data and file structure

Each sample has 2 individual .fq files per replicate. Sample names are included below.

AB_1_1 & AB_2_1 = stimulated optoFcR (in fig 5a, S4a)

AB_1_3 & AB_2_3 = stimulated control (in fig 5a, S4a)

AB_1_9 & AB_2_9 = M1 polarized (in fig 5b, S4b)

AB_1_10 & AB_2_10 = M2 polarized (in fig 5b, S4b)

AB_1_11 & AB_2_11 = stimulated optoFcR (in fig 5b, S4b)

AB_2_5 = unstimulated optoFcR (in fig 5a, S4a, S4b)

AB_2_7 = unstimulated control (in fig 5a, S4a, S4b)