Single cell assessment of trophoblast stem cell and primary cytotrophoblast derived organoids as placenta models

The establishment and function of the human placenta is dependent on specialized cells called trophoblasts. We here report transcriptomic data from n=3 pooled human first trimester patient-derived regenerative and n=3 pooled EVT differentiated trophoblast organoids (PD-TOrg) at single-cell resolution. This study sought to compare the undifferentiated and EVT-differentiated PD-TOrg molecular programs and cell states to those found in vivo and in hTSC-TOrgs previously published in Shannon et al., 2022. Placental tissues were collected following elective termination at the British Columbia Women's Hospital CARE clinic for PD-TOrg establishment. PD-TOrg cultures were established and differentiated as described and sequenced on either day 0 of culture (for regenerative organoid colonies), or day 7 of culture (for EVT-differentiated organoid colonies). Overall design: PD-TOrgs were established from 3 independent placental tissues using a modified version of previously described protocols and differentiated into EVT-trophoblast organoids by removal of WNT signaling components. Live trophoblasts were purified for mRNA sequencing from n=3 pooled regenerative organoid cultures on culture day 0 and from n=3 pooled EVT-differentiated organoid cultureds on culture day 7 in EVT-differentiation media using the 10X Genomics chromium single cell 3' reagent v3 chemistry kit.