Within- and across-generational effects of temperature: Exposure of Manduca sexta larvae to heat stress impacts future reproduction and offspring development

The effects of temperature on reproduction and other key fitness traits are often primarily considered only for the adult thermal environment, but exposure to thermal stress during earlier life stages may carry over to influence adult traits within a generation or even across generations. In this study, we assessed how an acute heat shock event experienced at two different points in Manduca sexta larval development (early and late) impacted adult performance and fitness traits and whether thermal exposure of parents elicited plastic changes in offspring traits. Heat stress during late larval development had significantly greater negative impacts on adult performance and fitness compared to earlier exposure. Adults that experienced a late larval heat shock failed to produce any viable offspring due to the complete elimination of egg hatching success. Larval heat stress during the parental generation reduced larval development times of their offspring in both control and heat shock condit..., , , # Within- and across-generational effects of temperature: exposure of *Manduca sexta* larvae to heat stress impacts reproduction and offspring development

[https://doi.org/10.5061/dryad.p8cz8w9w6](https://doi.org/10.5061/dryad.p8cz8w9w6)

## **Description of the data and file structure**

* **Ms_PE_Analysis.R** contains all R code used for data visualization and analysis

- **Ms_PE_indv_life_history_all.csv** contains data of individual life history measures (mass, development time, etc.) for both parental and F1 generations

* **Ms_PE_survival_gen1.csv** contains data of survival metrics (date and stage of individual death) for parental generation

- **Ms_PE_survival_gen2.csv** contains data of survival metrics (date and stage of individual death) for F1 generation

* **Ms_PE_fecundity_gen1.csv** contains data of fecundity metrics (numbers of eggs laid and hatch rates of eggs per moth pair) for parental generation

- **Ms_PE_fecundity_gen2.csv** contains data of fecundity metrics (num...,