Bioactivity Assay Protocols for Fermented Milk Metabolomics Study

This supplementary document provides detailed experimental protocols for all bioactivity assays conducted in the study titled "Predicting Functional Bioactivities in Fermented Milk Using Deep Learning on LC-MS Metabolomics." It includes methodological steps, calculation formulas, and references for antioxidant (DPPH, ABTS), enzymatic inhibition (α-amylase, α-glucosidase, ACE), and antiproliferative assays (HT-29 and MDA-MB-231 cell lines). These protocols support reproducibility and transparency in the evaluation of functional bioactivity in fermented dairy matrices.