Impact of depeltion of CG5694/ova on transcription in Drosophila ovaries [miRNA- and RNA-seq]. Impact of depeltion of CG5694/ova on transcription in Drosophila ovaries [miRNA- and RNA-seq]

Purpose: To analysis Ova impact on transcription in Drosophila ovaries by mRNA-seq and small RNA-seq Methods: mRNA-seq and small RNA-seq of RNA extracted from Drosophila ovaries Results: Ova has no obvious impact on protein coding genes expression or piRNA biogenesis. Overall design: Isolation of total mRNA from w1118(Ctrl) and germline-specific nosGAL4 driven ova RNAi ovaries, followed by deep sequencing analysis. Purification of total RNA from w1118(Ctrl) and germline-specific nosGAL4 driven ova RNAi ovaries using PEG8000 protocol. We extracted the small RNA according to proper size ( Seperation of 18-30 nt small RNA according to appropriate RNA markers), followed deep sequencing. We normalized the total reads number of small RNA from w1118(ctrl) and ova RNAi samples. After filtering the tRNA, snRNA, and miRNA, we got the small RNAs mapping to the repeat regions in Drosophila genome. For wild-type, it is usually a small peak at 21 nt representing endo-siRNAs and the rest peaks at 25 nt represented piRNAs. As it remains essentially unchanged in both w1118 and ova RNAi samples for both small RNA size distribution and abandunce (data provided in the smallRNA.txt), we conclude that Ova is not required for piRNA biogenesis.