RNA-seq expression profiling of terminal end buds and ducts from mouse mammary glands

The mammary glands of adult female mice were divided into ductal tissue and terminal end buds (TEBs). Basal and luminal epithelial cells were FACS sorted and RNA extracted for 75bp paired-end RNA-seq profiling using an Illumina NextSeq 500 sequencer. Overall design: Tissue was extracted from three independent mouse pools resulting in n=3 biological replicates for basal cells from ducts, basal cells from TEBs, and luminal cells from TEBs and n=2 biological replicates for luminal cells from ducts.