Table_2_Lactobacillus plantarum ZJUFB2 Prevents High Fat Diet-Induced Insulin Resistance in Association With Modulation of the Gut Microbiota.docx

Lactobacillus plantarum ZJUFB2 is a novel probiotic isolate derived from Chinese traditional sourdough that possesses promising probiotics properties. This study aimed to investigate the effects of L. plantarum ZJUFB2 (B2) on insulin sensitivity using mice fed on a high-fat diet (HFD) as well as to explore the involved mechanisms. Purposely, male C57BL/6 mice continuously received an intervention of B2 (~109 CFU/day) for 16 weeks. The results showed that B2 treatment remarkably ameliorated insulin resistance and hyperglycemia in HFD-fed mice. The B2 intervention significantly decreased the hepatic lipid accumulation, serum low-density lipoproteins cholesterol, and lipopolysaccharides, and regulated the bile acids levels as well as liver mRNA expression involved in lipid metabolism. Moreover, the B2 intervention significantly changed the gut microbiota, specifically, showed a lower abundance of obesity-related and inflammation-associated microbes, e.g., Ruminococcus and Mogibacteriaceae. Furthermore, it exhibited a higher abundance of short-chain fatty acids and bile salt hydrolas-producing bacteria, such as Bifidobacterium and F16 compared with the HFD group. The findings of this study suggested that B2 is a novel probiotic, effective in preventing insulin resistance by improving the gut microbiota and bile acids.

植物乳杆菌ZJUFB2是一种从中国传统发酵面食中分离得到的创新益生菌株，具有令人期待的益生菌特性。本研究旨在通过以高脂肪饮食（HFD）喂养的小鼠为模型，探讨植物乳杆菌ZJUFB2（B2）对胰岛素敏感性的影响，并深入探究其涉及的机制。有意为之，雄性C57BL/6小鼠连续接受B2（约109 CFU/天）的干预16周。结果显示，B2治疗显著改善了高脂肪饮食喂养小鼠的胰岛素抵抗和高血糖症状。B2干预显著降低了小鼠的肝脏脂质积累、血清低密度脂蛋白胆固醇和脂多糖水平，并调节了胆汁酸水平以及与脂质代谢相关的肝脏mRNA表达。此外，B2干预显著改变了肠道菌群，特别是降低了与肥胖相关和炎症相关微生物的丰度，例如瘤胃球菌和 Mogibacteriaceae 科微生物。进一步地，它增加了短链脂肪酸和胆盐水解酶产生菌的丰度，如双歧杆菌和F16，与高脂肪饮食组相比。本研究结果表明，B2是一种新型益生菌，通过改善肠道菌群和胆汁酸水平，有效预防胰岛素抵抗。