Early gene expression in mouse hematopoietic stem cells induced by Thrombopoietin stimulation and irradiation

Thrombopoietin (TPO) was shown to prevent irradaition-induced hematopoietic stem cells (HSCs) loss of function. A single injection of TPO to mice 45-60 min prior to irradiation is sufficient to reverse the long-lasting accumulation of persistent DNA damage. We used microarrays to analyze whether this effet can relie on TPO-induced specific transcriptional changes during this early time. 10,000 HSCs (Lin-Sca+Kit+-CD34-Flk2-) from pools of 8 to 10 C57BL/6 mice were FACS sorted and cultured in vitro for 45 min in medium containing or not TPO before irradiation. Microarrays were performed 45 min later.