Many cryogenic electron microscopy (cryo-EM) single particle analyses are constrained by the sample preparation step upon which aggregation, dissociation, and/or preferential orientation of particles

NIAID Data Ecosystem20

Huntingtin +/- DNA oligo cryo and negative stain grid set up and screening

冷冻电镜样品制备

亨廷顿蛋白结构功能

Huntingtin structure-function open lab notebook project.

NIAID Data Ecosystem20

Effects of miniemulsion operation conditions on the immobilization of BSA onto PMMA nanoparticles

纳米颗粒

蛋白质固定化

Abstract Polymer nanoparticles have been widely used in many biomedical applications, constituting a major incentive for immobilization of proteins. Poly(methyl methacrylate) nanoparticles were synthe

DataCite Commons2020-08-26 更新40

DataSheet1_Approaches to Using the Chameleon: Robust, Automated, Fast-Plunge cryoEM Specimen Preparation.docx

冷冻电镜样品制备

自动化蛋白质样本处理

The specimen preparation process is a key determinant in the success of any cryo electron microscopy (cryoEM) structural study and until recently had remained largely unchanged from the initial design

NIAID Data Ecosystem20

His-tagged beta galactosidase (LacZ) on a Ni-NTA lipid monolayer grid

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