Comparative proteomic profile in bee- and manually-collected <i>Taraxacum officinale</i> pollen

The study reports a first comprehensive approach to the proteome analysis done by comparing the protein profiles characteristic of bee- and manually-collected common dandelion (<i>Taraxacum officinale</i>) pollen. Proteins isolated from the bee- and manually-collected pollen was fractionated by two-dimensional electrophoresis, visualized by staining with Colloidal Coomassie G-250, and after tryptic-digestion analysed by mass spectrometry. A computer-assisted analysis was performed for comparison of protein profile maps typical of bee- and manually-collected dandelion pollen.