Comparative Analysis of Long Noncoding RNAs Expressed during Intramuscular Adipocytes Adipogenesis in Fat-Type and Lean-Type Pigs

The meat quality of local breed pigs
is more tender and juicier
than the imported varieties. The important reason is that the intramuscular
fat content is high. Even through modest sequence conservation and
evolution, the expression pattern and function of long noncoding RNAs
(lncRNAs) seem to be conserved. In spite of that, analysis of lncRNAs
associated with intramuscular fat development remains unknown to us
in porcine. Here, we systematically investigated lncRNAs of intramuscular
adipocytes of fat local Bamei pigs and lean Large White pigs to consider
the function of lncRNAs on intramuscular fat development. We selected
three piglets of both breeds separately to isolate intramuscular preadipocytes,
performed RNA sequencing across four stages (0, 2, 4, and 8 d) during
the intramuscular preadipocytes differentiation, and identified 1932
lncRNAs (760 novel). In addition, we have screened lnc_000414 closely
related to fat synthesis. This lncRNA function as an inhibitor in
the proliferation of porcine intramuscular adipocytes. These novel
findings will provide new targets for improving pork quality and making
pig breeding better.