RNA-binding protein RPS27 and gene expression regulation in human umbilical vein endothelial cells (HUVECs)

RPS27, a significantly down-regulated and validated RBP in kaposi's sarcoma (KS), and known to be associated with viral infection. As KS is an endothelial cell cancer, we used HUVECs as the cell model to explore the functions of RPS27. RNA-seq technology was used to investigate the differentially expressed genes in HUVECs. By using small interference RNAs (siRNAs) to silence the expression of RPS27 (siRPS27), we detected significant decrease of RPS27 expression in RNA levels comparing with the negative control (siNC) samples. Overall design: Comparative gene expression profiling analysis of RNA-seq data for RPS27 gene silent HUVECs. The experiment was divided into two groups, each with three replicates. NC group: HUVECs transfection 0.1µM RPS27 siRNA NC; RPS27 siRNA group: HUVECs transfection 0.1µM RPS27 siRNA.