Next Generation Sequencing (NGS) for quantitative transcriptomic analysis of Francisella strains (Francisella novicida, ribD-deficient Francisella novicida , Francisella tularensis)

To understand differences of gene expression profiles between Francisella strains RNA profiles of Francisella strains were generated by deep sequencing, in triplicate, using NovaSeq6000. qRT–PCR validation was performed using SYBR Green assays. Our study represents the first detailed differential transcriptomic analysis of Francisella strains , with biologic replicates, generated by RNA-seq technology. Overall design: mRNA profiles of Francisella strains were generated by deep sequencing, in triplicate, using NovaSeq6000.