Summary of immunofluorescence-based colocalization studies of HCV proteins and cellular marker proteins.

Naïve high-passage Huh-7 cells were infected with 30 TCID50/cell of Jc1 and 48 h later processed for indirect immunofluorescence. Signal intensities were quantified by using Intensity Correlation Analysis (ICA) of the Image J software package and are given as Pearson's correlation coefficient (PCC). PCC can range from −1 to 1, corresponding to no or perfect colocalization. Only values higher than 0.5 were considered as colocalization.n.a., not applicable owing to incompatible antibodies.ADRP, adipose differentiation-related proteins or adipophilin; CLIMP-63, cytoskeleton linking membrane protein 63; COP I, coat protein I; COP II, coat protein II; EEA1, early endosome antigen 1; EH, epoxide hydrolase; ERGIC-53, ER Golgi intermediate compartment; GOS-28, Golgi SNARE protein 28; GRP94, glucose related protein 94; Lamp-3, lysosome-associated membrane protein-3; LBPA, lysobisphosphatidic acid; LC-3, light chain protein 3; PDI, protein disulphide isomerase; Rab5, Ras-related GTP binding protein 5; Rab7A, Ras-related GTP binding protein 7A; Rab11, Ras-related GTP binding protein 11; Rab21, Ras-related GTP binding protein 21;. sec13, secretory protein 13; TGN46, Trans Golgi network protein 46.