Analysis of metabolic characteristics of peripheral blood mononuclear cells in patients with leukemia at the single cell level

We collected peripheral blood from leukemia patients at different stages, including those newly diagnosed as untreated, as well as those in remission on therapy, with a subset of samples including peripheral blood and bone marrow samples. First, mononuclear cells were isolated from the collected peripheral blood, followed by single-cell RNA sequencing and follow-up sequencing results for subgroup classification, focusing on changes such as glycolipid metabolism of B cells as well as T cells. The aim of this project was to explore the changes in metabolic remodeling in leukemia patients with the aid of single-cell technology to provide assistance in the diagnosis and treatment of the disease. The focus of this study was to collect peripheral blood from leukemia patients at different stages of disease onset, followed by single-cell RNA sequencing of peripheral blood mononuclear cells, combined with clinicopathological and other relevant data, to investigate changes in metabolic remodeling in leukemia patients. This project is a study of observational nature and does not involve modification of the treatment regimen to cause adverse effects to patients.