PCR-free Illumina short reads of 61 Arabidopsis thaliana accessions to estimate genome size and the contribution of satellite repeats. The relationship between genome size and major satellite repeats in Arabidopsis thaliana

In a companion study (PRJEB73474), we determined that 27 chromosome-level Pacbio CLR assemblies of Arabidopsis thaliana accessions are all ∼120 Mb in size. This is noteworthy because we have previously estimated by flow cytometry that A. thaliana genomes vary more than 10% in genome size. Regardless of the sequencing technology, it is still extremely challenging to assemble major satellite repeats. Therefore, we employed k-mer and BLAST-based approaches in PCR-free Illumina short reads to establish the relationship between genome size and three major satellite repeats, namely centromeres, 45S and 5S rDNAs. In this project, we release PCR-free Illumina short-reads of 61 A. thaliana accessions to complement data published in the above-mentioned study.