Single cell RNA (scRNAseq) and T cell receptor sequencing (scTCRseq) of cerebrospinal fluid (CSF) samples from three patients with spinal cord injury (SCI) [TCR-seq]

The CSF encompasses a highly controlled immune system. However, little is known about the alterations in CSF immune microenvironment after SCI. Here, we performed scRNA and TCRseq on CSF collected from three SCI patients caused by car accidents to analyze the transcriptomic changes of CSF immune cells. The CSF of three SCI patients were isolated via lumbar puncture within 48 hours after injury. Following red blood cell lysis and magnet-activated cell sorting (MACS) for live singlets, CSF samples were then analyzed by scRNAseq and scTCRseq.