Data from: De novo discovery and multiplexed amplification of microsatellite markers for black alder (Alnus glutinosa) and related species using SSR-enriched shotgun pyrosequencing.

Recent developments in sequencing technologies and bioinformatics analyses
provide an unprecedented opportunity for cost and time effective high
quality microsatellite marker discovery in non-model organisms for which
no genomic information is available. Here, we use shotgun pyrosequencing
of a microsatellite-enriched library to develop, for the first time,
microsatellite markers for Alnus glutinosa, a keystone tree species of
European riparian woodland communities. From a total of 17,855 short
sequences, we identified 590 perfect microsatellites from which 392 had
designed primers. A subset of 48 loci were tested for amplification,
twelve of which were polymorphic in A. glutinosa. These twelve loci were
successfully co-amplified in a single multiplex PCR experiment and
validated for population genetics applications. In addition, ten and eight
of these microsatellites were found to be transferable to the related A.
incana and A. cordata species. The developed multiplex of 12
microsatellite markers therefore provides new opportunities for
experimental evolutionary and forest genetics research in Alnus.