RNA-Seq analysis of whole cell, cytoplasmic and nuclear fractions from GANP depleted cells and control cells

RNA-Seq was performed to identify cellular mRNAs that are dependent on GANP, a TREX-2 complex component, for their nuclear export. The GANP gene was endogenously fused with HA and AID-tags in DLD-1 cells. The GANP protein was rapidly degraded using the AID-tag system by incubating cells in the presence of auxin for 8 h. RNA-Seq was performed using RNA from whole cell, cytoplasmic and nuclear fractions from GANP depleted cells or control cells. Samples were sequenced on the Illumina HiSeq 2500 with paired end 150 bp reads.