Effects of pegylated hyaluronidase (pegHYAL) and Spiperone treatment on content of MSCs in the lungs derived from C57BL/6 mice with the irreversible pulmonary.

The content of MSCs (% of all CD45- -cells) in the lung of C57BL/6 mice on the 21 day of the experiment after single dose intratracheal instillation of BLM. Lung-derived MSCs were labeled with antibodies against CD45, CD44, CD73, CD90, CD106 antigens and analyzed by flow cytometry. Our data imply that on the 21st day of the experiment lung CD45- -cells of mentioned above groups mice expressed on the surface of CD44, CD73, CD90, and CD106. The all of these populations is sorted from one sample. Dot plots are representative figures of three independent experiments with the mean percentage of at least three experiments.
Results are presented as mean and SEM.
*—significance of the difference with the mice, that received intratracheal 0.9% NaCl (P <0.05),
&—significance of the difference with the mice, that received intratracheal BLM and treated with 0.9% NaCl (P<0.05), t test was used.
Effects of pegylated hyaluronidase (pegHYAL) and Spiperone treatment on content of MSCs in the lungs derived from C57BL/6 mice with the irreversible pulmonary.