High-throughput discovery of transmembrane helix dimers from human single-pass membrane proteins with TOXGREEN sort-seq

The oligomerization of the transmembrane helices of single-pass membrane proteins is crucial to biological function, and its misregulation can lead to many diseases. The study of transmembrane helix oligomerization is facilitated by the availability of genetic reporter assays, which are essential tools for understanding the organization and biology of single-pass systems. In particular, reporter assays are crucial for mapping the oligomerization interfaces of transmembrane helices through scanning mutagenesis, but their application is limited by the need to clone and measure each construct individually. Here, we present “TOXGREEN sort-seq”, a high-throughput version of the TOXGREEN assay that enables the direct measurement of transmembrane helix oligomerization in large libraries using fluorescence-activated cell sorting and next-generation sequencing. We show that TOXGREEN sort-seq is robust and reproduce the direct measurements of individual constructs with good accuracy and sensitivi..., , # High-throughput discovery of transmembrane helix dimers from human single-pass membrane proteins with TOXGREEN sort-seq

Dataset DOI: [10.5061/dryad.qnk98sfv8](10.5061/dryad.qnk98sfv8)

This repository contains:

* Structural model of transmembrane helix dimers (PDB files)
* Sort-seq Data
* NGS data processing script (with example input data files)

## Structural model of transmembrane helix dimers (PDB files)

### Description of the data and file structure

The files include the CATM and AlphaFold3 (AF3) models of the twelve GAS-right dimers validated by the mutagenesis. The files also include the CATM and AF3 models of the three proteins whose mutagenesis matches an AF3 model but not a CATM model. The CATM and AF3 models have been aligned with each other based on the transmembrane helices.

#### Name scheme:

``<Uniprot Code><protein name abbreviation><modeling program><model number><.pdb>``

#### Files of proteins matching the CATM model:

* `O75056_syndecan-3_AF3_model_0.pdb`
* `O...,