Monolayer behaviour of DMPC and DEHP with cholesterol

Donated blood is typically stored in bags made of PVC plasticized with DEHP. DEHP migrates into the blood and the amount in the blood cells can be 5 to 10% of the phospholipid content. This stabilizes the membranes of red blood cells and increases the shelf life of stored blood. DEHP is toxic and a non-toxic alternative is desired. We now plan to extend previous studies on a two-component system of the lipid, DMPC with DEHP by adding cholesterol. We will investigate the role of DEHP in a system with more relevant composition where it causes different behaviour. Monolayers will be investigated at an air-water interface using specular reflectivity. Insights into stabilization will lead to greater understanding of how to preserve blood cells and increase the storage life of donated blood.