Figure S2 - Open Reading Frame 3 of Genotype 1 Hepatitis E Virus Inhibits Nuclear Factor-κappa B Signaling Induced by Tumor Necrosis Factor-α in Human A549 Lung Epithelial Cells

HEV ORF3 suppressed TNF-α induced NF-κB in Huh7 cells. Expression and localization of pORF3 in Huh7 cells. Huh7 cells were transfected with either pORF3-GFP (ORF3) or pEGFP-N1 (GFP) for 48 h, the ORF3 protein with green fluorescent was observed with laser confocal microscopy (magnification: 1000×). (B) Huh7 cells were transfected with either pORF3-GFP (ORF3) or pEGFP-N1 (GFP) for 48 h, stimulated with TNF-α (50 ng/ml) for 6 h, and then subjected to western blotting, using the untreated cells as a control (UN). (TIF)